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This study investigated the impact of improvised organic reagents on senior secondary school students’ level of motivation in chemistry. Two research questions guided the study and three hypotheses were formulated and tested at 0.05 level of significance. The research design was a quasi-experimental pretest-posttest non-randomized control group. The sample included 120 Senior Secondary School Three (SS3) chemistry students randomly drawn from four co-educational schools in the Onitsha Education Zone of Anambra State, Nigeria. The instrument used was Students’ Motivational Level in Chemistry Questionnaire (SMLCQ) which was developed and validated for data collection. An internal consistency estimate of 0.82 was obtained using Cronbach’s alpha procedure. The data generated from the study were analyzed using mean and standard deviation to answer the research questions; two-way analysis of variance and two-way analysis of covariance was used to test the null hypotheses. Results of the findings indicated that improvised organic reagents promote the motivational level of chemistry students. It is recommended that teachers of chemistry should use improvised organic reagents in the teaching of chemistry to increase students’ enthusiasm in learning chemistry.